THE EFFECT OF MORINGA LEAF EXTRACT (MORINGA OLEIFERA L) AGAINST MOTILITY OF SPERMATOZOA MICE EXPOSED TO MONOSODIUM GLUTAMATE
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Abstract
Objective: Excessive use of MSG in everyday life can cause infertility to men. Exposure to MSG can cause cells to experience oxidative stress which subsequently triggers the generation of free radicals. Free radicals can be resisted with antioxidants. Material & Methods: This research was a laboratory experimental test with a post-test only control group design using male mice as the experimental object. The statistical test used the One Way ANOVA test. Results: In this research, 5 male mice were assigned into 4 groups and 1 control group. Using the One Way ANOVA test, it was found that there were significant differences in ≥ 2 treatment groups in the motility group of spermatozoa with active movement (p < 0.001) and the motility group of spermatozoa with weak movement (p = 0.036). The two groups showed significant differences. The Post hoc LSD test in the motility group with active movement showed significant differences between the control group with MSG treatment (p < 0.001) and the treatment group given Moringa leaves extract of 600 mg/kgW (p < 0.001) and 1200 mg/kgW (p < 0.001). Conclusion: Moringa leaves extract can effect the improvement of the motility of spermatozoa exposed to monosodium glutamate at a given dose of 300 mg/kgW and 600 mg/kgW.
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Spermatozoa motility, moringa leaves extract, monosodium glutamate, free radical
Farida N. Monosodium glutamate dangerous for the body. 2018. [cited 2020 Feb 26]. Available from: http://yankes.kemkes.go.id/read-berbahayakah-mgs-bagi-tubuh-5679.html
Widayati E. Biological Oxidation, Free Radicals, and Antioxidants. Maj Ilm Sultan Agung. 2012; 50 (128): 26–32.
Winarsi H. Natural Antioxidants and Free Radicals, Their Potential and Application in Health. 5th ed. Yogyakarta: KANISIUS (Member of IKAPI); 2011.
Sikka SC. Role of Oxidative Stress and Antioxidants in Andrology and Assisted Reproductive Technology. J Androl. 2010; 25 (1): 5–18.
Saputri AA, Juniarto A. Effect of Soybean Extract (Glycine max) on Sperm Motility of Balb/c Male Mice. Faculty of Medicine, Diponegoro Univ, Semarang. 2007; 1 (1): 1–8.
Nasihun. Non-surgical therapy in infertility. Medical Science; 2001.
Irawan D. HI. obesity and sperm quality. Indonesian Medical Journal; 2008.
Jóźków P, Rossato M. The Impact of Intense Exercise on Semen Quality. Am J Mens Health. 2017; 11 (3): 654–62.
Putri GS, Romdhoni MF, Bahar Y. Effect of Ethanol Extract of Basil Leaves on Ureum and Creatinine Levels of Male Wistar Rats (Rattus norvegicus, Wistar Strain) induced by Monosodium Glutamate. Herb-Medicine J. 2019; 2 (1): 12–9.
Sukmaningsih, Ermayanti IGAM, Wiratmini NI, Sudatri NW. Spermatogenesis disorders after administration of monosodium glutamate in mice (Mus musculus L.). J Biol. 2009; XV (2): 49–52.
Iswara I, Yonata A, MP Doctor, Faculty Medicine, Lampung University, Science B, et al. Toxic Effects of Monosodium Glutamate Consumption Toxic Effects Consumption of Monosodium Glutamate. Majority. 2016; 5 (September): 100–4.
Arifin B, Ibrahim S. Structure, Bioactivity and Antioxidant Flavonoids. J Zarah. 2018; 6 (1): 21–9.
Prayitno SA, Kusnadi JK, Murtini ES. Characteristics (Total Flavonoids, Total Phenol, Antioxidant Activity) Extract of Red Betel Leaf Powder (Piper crocatum Ruiz & Pav.). Foodscitech. 2018; 1 (2): 26.
Verma, AR, Vijayakumar, M., Mathela, CSRao C. In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. 2009.
RI D. General Standard Parameters of Medicinal Plant Extracts. 2000.
Harborne. JB Phytochemical Methods: A Guide to Modern Methods of Analyzing Plants. III. Bandung: Bandung Technology Institute ; 2008.
Suparni. The effect of vitamin c on sperm count and sperm morphology of adult male mice (mus musculus) exposed to monosodium glutamate. 2009; 1–83.
Sukmaningsih aa S., Ermayanti IGAM, Wiratmini NI, Sudatri NW. Spermatogenesis disorders after administration of monosodium glutamate in mice (Mus musculus L.). J Biology. 2009; XV (2): 49–52.
Kadir RE, Kadir RE, Omotoso GO, Balogun TJ, Oyewopo AO. Effects of Monosodium Glutamate on Semen Quality and the Cytoarchitecture of the Testis of Adult Wistar Rats. 2011; 7 (1): 39–46.
Hazani K. Effect of ethanol extract of Moringa oleifera L leaves on levels of Malondialdehyde (MDA) and quality of epididymal spermatozoa of mice (Mus musculus L) exposed to lead (Pb). Maulana Malik Ibrahim State Islamic University Malang; 2014.
Kasolo JN, Bimenya GS, Ojok L, Ochieng J, Ogwal-Okeng JW. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plants Res. 2010; 4 (9): 753–7.
Prayoga PR. The Effect of Tomato (Lycopersicum esculentum mill) to Amount, Motility, and Morphology of Spermatozoa in Cigarettes-induced Infertility Patients. 2015; 4: 60–6.
Nasihun T. Role of ROS on Spermatozoa Function. Indonesian Andrology Journal. 2012.
Lauralee S. Human Physiology: from cells to systems. 8, editor. Medicine book publisher EGC; 2012.
Biology Viability ON. Motility and Viability of Mice (Mus Musculus L.) Spermatozoa After Giving Temu Putih Extract (Curcuma Zedoaria (Berg.) Roscoe.). J Biol Udayana. 2010; 14 (1): 20–3.
Herdriningrat S. The effect of infusion of young mangosteen fruit on spermatozoa of mice. Indonesian Andrology Magazine; 2002.
Adnan. Effect of pacing rhizome extract on mice fertility. Makassar: Makassar State University; 2000. 1–10 p.
Sinha Hikim AP, Lue YH, Wang C, Reutrakul V, Sangsuwan R, Swerdloff RS. Posttesticular antifertility action of triptolide in the male rat: Evidence for severe impairment of cauda epididymal sperm ultrastructure. Journal Andrology. 2000; 21 (3): 431–7.
Susetyarini E. The number of spermiogenesis cells of white mice given beluntas leaf tannins (Pluchea Indica) as a learning resource. Biology Science, Environment, and Learning. 2011; 20 (5): 562–466.