THE EFFECT OF NIFEDIPINE ON SURVIVE CELL, APOPTOTIC CELL AND NECROTIC CELL OF IPSILATERAL TESTICULAR GERMINAL EPITELIAL CELLS ON MALE WISTAR RATS WITH UNILATERAL TESTICULAR TORSION

##plugins.themes.bootstrap3.article.main##

##plugins.themes.bootstrap3.article.sidebar##

PDF
Published 2019-07-02
Andrie Rhomdhon Kurniawan Lukman Hakim Doddy M Soebadi

Abstract

Objective: This study aimed to compare the number of survival, apoptotic and necrotic cells of ipsilateral testicular germinal epithelial cells in male wistar rats with unilateral testicular torsion between nifedipine given and control groups. Material & Methods: Thirty male wistar rats aged 10-12 weeks were randomly divided into 5 groups, each consisted of 6 rats. The negative control group (KN) underwent a sham procedure and left orchidectomy. Positive control group 4 (KP4) and 10 (KP10) performed left torsio testis 3 x 360 degrees medially for 4 hours and 10 hours respectively, then performed orchidectomy 4 hours after detorsion. The 4-hour (N4) and 10 hours (N10) nifedipine treatment group received the same treatment with positive control, but 30 min before detorsion performed, nifedipine were given intraperitoneal 100μg/kg. Within 1 hour after orchidectomy, cell count was calculated using flow cytometry. Results: It was found that the 4 (N4) and 10 hours (N10) nifedipine treatment group had a higher survival cells and also a lower number of apoptotic and necrotic cells compared to the positive control group. It was found that the 10 hours nifedipine treatment group (N10) had a lower number of apoptotic and necrotic cells compared to the 10 hour positive control group (KP10). The difference was statistically significant with p value <0.05. However, in KP4 and N4 group compared with KP10 and N10 group, higher apoptotic cells was obtained. This was a new phenomenon that needs to be investigated more deeply. Conclusion: Intraperitoneal administration of nifedipine prior to testicular detorsion may reduce the number of apoptotic and necrotic cells of testicular germinal epithelial cell, and may increase the number of survival cells in ipsilateral testes with unilateral testicular torsion.


##plugins.themes.bootstrap3.article.details##

Keywords

Survive cell, apoptosis, necrosis, nifedipine, unilateral testicular torsion, flow cytometry

References

Ellati RT, Kavoussi PK, Turner TT, Lysiak JJ. Twist and shout: A clinical and experimental review of testicular torsion. Korean J Urol. 2009; 50(12): 1159–67.

Lee SM, Huh JS, Baek M, Yoo KH, Min GE, Lee HL, et al. A nationwide epidemiological study of testicular torsion in Korea. J Korean Med Sci. 2014; 29(12): 1684–7.

Purnomo BB. Kelainan pada testis. In: Dasar-Dasar Urologi. 3rd ed. Jakarta: Sagung Seto; 2011. p. 20–4.

Nurhadi P, Soetojo. Profil akut skrotum di RSUD Dr Soetomo Surabaya periode Januari 2011-Desember 2015. 2015.

Wampler SM, Llanes M. Common scrotal and testicular problems. Prim Care-Clin Off Pract [Internet]; Elsevier Ltd. 2010; 37(3): 613–26. Available from: http://dx.doi.org /10.1016/j.pop.2010.04.009

Xoe Gao, Hannrui Zhang, Souad Belmadani, Cunxi Wu, Xiangbin Xu, Howard Elford, et al. Role of TNF-induce reactive oxygen species in endothelial dysfunction during reperfusion injury. Department of Internal Medicine, Medical Physiology and Pharmacology, and Nutritional Science, Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri. Amj Physiol Heart Circ Physiol. 2008; 295: H2242-H2249.

Carmelo Romeoa, Pietro Impellizeria, Teressa Arrigob, Pietro Antonuccioa, Marriella Valenziseb, Sylviomirabellib, et al. Late hormonal function after testicular torsion. A Department of Medical and Surgical Pediatric Science, Unit of Pediatric Surgery, University of Messina, 98124 Messina, Italy; 2009.

Filippo De Lucab Smith’s. General Urology. 17th Edition. The Mcgraw Hill Companies. University of California School of Medicine, Department of Urology, San Francisco, General Hospital, San Fransisco, California; 2008.

Alif S. Torsio testis di RSUD Dr. Soetomo Surabaya, studi prospektif selama dua tahun. Surabaya: Fakultas Kedokteran Universitas Airlangga; 1991.

Campbell Walsh. Urology, 10th edition. Penn Medicine/University of Pensylvania Health System. Perelman Centre of Advance Medicine Philadelphia, Pensylvania; 2012.

Callewaert PRH, Van Kerrebroeck P. New insights into perinatal testicular torsion. Eur J Pediatr. 2010; 169(6): 705–12.

Majno G, Joris I. Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol [Internet]. 1995; 146(1): 3–15. Available from: http://www.pubmedcentral. nih.gov/articlerender.fcgi?artid=1870771&tool=pmcentrez&rendertype=abstract.

Saba M, Morales CR, Lamirande D, Gagnon C. Morphological and biochemical changes following acute unilateral testicular torsion in pre pubertas rats. J Urol. 1997; 157: 1149–54.

Collard CD, Simon Gelman. Pathophysiology, clinical manifestations, and prevention of ischemia–reperfusion injury basic pathophysiology of ischemia–reperfusion injury. Anesthesiology. 2001; 94(6): 1133–8.

Hardjowijoto S. The effects of unilateral testicular torsion upon immunity modulation and apoptosis of germinal cells in the contralateral testis. Folia Medica Indones. 2005; 41(1): 9–17.

Krishnamurthy H, Weinbauer GE, Aslam H, Yeung CH, Nieschlag E. Quantification of apoptotic testicular germ cells in normal and methoxyacetic acid‐treated mice as determined by flow cytometry. Journal of Andrology. 1998; 19(6): 710-7.

Metrovi J, Drmi-Hofman I, Pogoreli Z, Vilovi K, Supe-Domi D, Ana Seselja-Perisin VC. Beneficial effect of nifedipin on testicular torsion-detorsin injury in rats. Urology. 2014; 84(5): 1194–8.

Dahlan S. Statistik untuk kedokteran dan kesehatan: uji hipotesis. Jakarta: Bina Mitra Press; 2006.

Clapper DL, Walsethv TF, Dargies PJ, Cheung H, Ii L. Pyridine nucleotide metabolites stimulate calcium release from sea urchin egg microsomes desensitized to inositol trisphosphate. J Biol Chem Biol Chem. 1987; 262: 9561–8.

Higuchi M, Honda T, Proske RJ, Yeh ET. Regulation of reactive oxygen species-induced apoptosis and necrosis by caspase 3-like proteases. Oncogene. 1998; 17(21): 2753.

Section
Articles
Copyright Information
Department of Urology, Faculty of Medicine/Airlangga University